Crystallin promoter

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August undefined, 2024

WebBy use of a gene fusion of the alpha A-crystallin promoter to the viral immortalizing oncogene, polyoma large T antigen (PyLT), we created transgenic mice that express PyLT specifically in ocular lens. Expression of large T antigen during embryonic development led to a perturbation in lens development, specifically, an interference with the ... WebThe human papillomavirus type 16 (HPV-16) E6 and E7 oncogenes are thought to play a role in the development of most human cervical cancers. These E6 and E7 oncoproteins affect cell growth control at least in part through their association with and

Deletions at the Cryge promoter. (a) Deletion constructs.

WebCrystallin promoters of widely different species are dependent on the same suite of transcription factors and function in similar ways in lens-cells of other species both in culture and in transgenic models (for example: [31–33]). This allows comparison of promoters from both chicken and mouse in mouse cells. WebA conserved and essential DNA motif in the promoters of the γ-crystallin genes is the binding site of the SOX proteins that activate the promoters (Kamachi et al., 1995). … razorbacks basketball ncaa tournament

Characterization of the chicken L‐Maf, MafB and c‐Maf in crystallin ...

WebDec 3, 2013 · CpG island methylation in the αA-crystallin promoter was similar in age-related cataract and high-myopic cataract classified as Lens Opacity Classification System III Grade Nuclear Color 2–3. Pyrosequencing, a real-time DNA sequencing method, was used to determine single-base variations caused by CpG methylation of the CRYAA … WebEarlier experiments showed that the betaB1-crystallin promoter sequence from -120 to -48 contains a cis element (PL2 at -90 to -76) that stimulates the activity of a heterologous promoter in lens cells but not in fibroblasts. WebLens-specific expression and developmental regulation of the bacterial chloramphenicol acetyltransferase gene driven by the murine alpha A-crystallin promoter in transgenic … razorbacks cross country

Aberrant TGF-β1 signaling activation by MAF underlies ... - Nature

Sequence determinants of human gene regulatory elements

WebDec 29, 2008 · The scallop Ω-crystallin promoter sequence contains an abundance of motifs that are similar to those in vertebrate crystallin promoters. These include cis … WebThe beta-B1 crystallin promoter produced GFP expression in the lenses of 37% of injected embryo’s after 48 hours. Fifty percent of MO injected … razorbacks cotton bowlWeb76 of this system. Data on crystallin promoter activity would also facilitate the expression 77 of introduced proteins in zebrafish lens and other tissues. 78 79 A comparison of mouse and zebrafish -crystallin promoter activity can also help detail 80 the evolution of tissue specific expression. Past studies have examined the evolution of razorback screensaver

"WebFeb 21, 2024 · The sequence between SacI and AfeI was replaced with a sequence containing CG-depleted chicken lens δ1-crystallin gene (Sasaki) promoter 51, a … " - Crystallin promoter

Crystallin promoter

Characterization of the chicken L‐Maf, MafB and c‐Maf in crystallin ...

WebMay 4, 2006 · Dramatic upregulation of αA-crystallin expression is associated with primary lens fiber cell differentiation. Previous studies have shown that a −366 to +46 αA … WebI am a highly motivated and passionate individual with a talent for organization and project management. I have enjoyed leading teams to complete projects in various industries …

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Webgene-specific sequences such as the gene-specific promoter sequence in the proximal promoter of the small heat shock protein gene, alpha B-crystallin (cryab) , may have critical roles in regulating gene-specific activity from eukaryotic promoters. AlphaB-crystallin is a novel regulator of brain metastasis in triple-negative breast cancer. WebMar 1, 1989 · Recently Landel et al. (1988) have combined the α -crystallin promoter with the ricin cytotoxic gene to produce a single line of transgenic mice with a somewhat more severe phenotype consisting of a still smaller lens and other interesting eye malformations.

WebSep 1, 2002 · These data suggest that the chicken β B1-crystallin promoter is the promoter of choice to direct the high level ectopic expression of RNAs to lens fiber cells … WebThis study concluded that there existed a TGF-β responsive element in the promoter just 285 base pairs upstream of the transcriptional start site ... porcine TGF-β1 under the lens-specific αA-crystallin promoter and then another similar line was created but with the gene driven by the βb-crystallin promoter, corresponding to another lens ...

WebJul 1, 2002 · It is proposed that Pax6 occupies and represses the chicken βB1-crystallin promoter in lens epithelial cells, and is displaced by Prox1 and Mafs, which activate the promoter, in differentiating cortical fiber cells. ... It is shown that the transactivation potential of c-Maf and MafB for the rat gammaD-crystallin Maf-responsive element (gammaD ... WebMar 1, 2003 · In order to study lens-retina relationships during development, we cloned the zebrafish alphaA-crystallin cDNA and its promoter region. Using a 2.8-kb fragment of the zebrafish alphaA-crystallin promoter (z (alpha)Acry), we expressed the diphtheria toxin A fragment (DTA) in zebrafish embryos in a lens-specific manner.

WebWe have further compared the γD-crystallin promoter with the γB-crystallin promoter, the promoter with the most extended developmental expression. We show that the γ …

WebFGF2 strongly activates expression of the αA-crystallin gene promoter coupled with the DCR1 enhancer compared to the promoter alone. 234 Interestingly, FGF signaling also regulates expression of a large set of miRNAs that modulate gene expression during FGF2-induced lens fiber cell differentiation. 157 At least three miRNAs, including miR-143 ... razorbacks coaching staffWebDec 1, 2012 · The gene promoter contains response elements for some key transcription factors essential for normal lens development. Prominent among these are factors such as Pax6, c-Maf, Sox2, Prox1, and Six3 [93,94]. In many crystallin gene promoters, elements for lens and non-lens expression are combined in the same 5 ′ region. simpsons dvd season 17WebJan 15, 2000 · Many of the crystallin promoters have been carefully dissected into component cis -acting elements with critical regions and putative transcription factor binding sites identified ( Cvekl and Piatigorsky, 1996; Duncan et al., 1996; Matsuo et al., 1991 ). razorbacks calling the pigsWebThis present study is the first to show that mammalian α-crystallin promoter function can be analyzed in zebrafish embryos by observing green fluorescent protein (GFP) … simpsons dvd season 11WebRetrospectively from the results of ( Maf) knock-out mice. it was also shown that MAF binds the promoter sequences of some, B-crystallin promoters ( Ring et al., 2000 ). Three … simpsons dvd season 12WebCrestline serves sophisticated institutions, family offices and high-net-worth individuals in North America, Europe and Asia who seek innovative alternative investment solutions … simpson sdwc15450WebApr 8, 2024 · Further explorations unveiled the increased MAF in high myopia and its participation in a direct activation of crystallin promoters and an indirect activation via … razorbacks crib bedding