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WebTable 3. Recommendations for transformation of ligation mixture Tranformation method Treatment of the ligation mixture before transformation Volume of the WebMay 3, 2013 · PCR-purified products were cloned using a ClonJET™ PCR cloning kit (Fermentas, Canada). Sequencing and phylogenetic analysis. DNA sequencing of cloned products was performed in both directions by a DNA service company (Source BioScience Co., UK). Internal primers were designed for each of the polymerase genes (primer … WebThe CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules … changes in inventories of finished goods 意味