WebThe cDNA was synthesized using Biofact RT kit (BioFACT™, Korea) according to the manufacturer’s standard protocol. A two-step qRT-PCR reaction was performed using 2× Real-Time PCR Master mix (including … Web연구의 발전을 넘어, biofact는 인류의 진보를 약속합니다. ‘미래 바이오 산업’ 기술혁신을 추구하는 기업 인류의 지속성장을 위한 밑거름이 될 것을 약속합니다.
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WebIn archaeology, a biofact (or ecofact) is any organic material including flora or fauna material found at an archaeological site that has not been technologically altered by … WebformedwithacDNAsynthesiskit(Biofact,Korea)according tothemanufacturer’sinstructions.Polymerasechainreac-tion(PCR)wasperformedusinggene-specificprimersand SYBR Green kit (Biofact, Korea). Following primers were used;Glyceraldehyde3-phosphatedehydrogenase(GAPDH)
WebMar 15, 2024 · K. brevifasciata was collected from the Sinpyeongcheon Stream under a collection permit issued by the Ministry of Environment in Korea. K. brevifasciata genomic DNA was extracted from the caudal fin using the Genomic DNA Prep Kit (Biofact, Korea), and the extracted genomic DNA was stored at Soonchunhyang University. The complete … WebBioFact™ 2X OneStep Multiplex qRT-PCR Kit for Probe is the best optimized to a system which is using sequence specific designed probe from various RNA samples. The …
WebJun 9, 2024 · The real-time polymerase chain reaction was performed using BioFact™ 2X Real-Time PCR Master mix kit (BIOFACT) and the StepOne Plus™ quantitative Real time PCR detection System (Applied Biosystems). ... staining with EcoDye™ DNA solution (BIOFACT, Korea), and Finally, under UV light the gel image was captured in E-Box gel … WebFeb 8, 2024 · Each 20-µl reaction mixture contained 2–4 µl template DNA (100–150 ng), 1× F-star Taq Reaction buffer (BioFact, Korea), 0.2 mM of dNTP (BioFact, Korea), 10 pmol of both primers, 1.25 U of F-star Taq DNA polymerase (BioFact, Korea) and 1 µl dimethyl sulfoxide (Sigma, St Louis, USA). Thermal cycling was conducted on an Applied …
WebIn biology, a biofact is dead material of a once-living organism. [1] In 1943, the protozoologist Bruno M. Klein of Vienna (1891–1968) coined the term in his article …
WebBIOFACT Co.,Ltd. - Korea supplier of Taq, PCR, DNA Prep Kits, DNA Amplification, DNA Purification, DNA isolation, DNA Clean Up, Taq DNA Polymerase, Multiplex PCR, … high waisted safari shortsWebJun 10, 2024 · We performed PCR using BioFACT™ Taq DNA Polymerase (Biofact, Daejeon, Korea). The 25 µL PCR mixture was composed of 5 µL of 10× DNA polymerase buffer, 2.5 units of 10× Taq DNA polymerase, 1 µL of genomic DNA, 10 pmol of each primer, and 0.5 µL of a 0.2 M dNTP mixture. The PCR conditions were as follows: denaturing at … high waisted sailor culottesWebFeb 28, 2024 · All qRT- PCR analyses were performed CFXrealtime PCR system CFXsystem software (Bio-Rad, CA) using Ever-Green 20 fluorescent dye (BioFACT, Daejeon, Korea). examineIbINH gene expression profiles, various tissues including leaf (5th from top),stem (4th-6th inter- node), fibrous root, pigment root storageroot were … s malloryWebPrepare your Skin for Summer! We offer next day delivery on products purchased before 12 noon! Delivery costs are €5 anywhere in Ireland. This charge is for each order not each product. So whether you are buying 1 … s m electricsWebBioFact Genomic DNA Prep Kit [Column Type](id:8637443), View quality DNA Preparation, Prep kit, DNA isolation details from BIOFACT Co.,Ltd. storefront on EC21.com. Buy best BioFact Genomic DNA Prep Kit [Column Type] with escrow buyer protection. ... BIOFACT Co.,Ltd. [Korea] Address 2F/3F, Guk-je Building, EXPO ST.240, Yuseong-Gu … high waisted sailor button pantsWebBioFact 2X Multi-Star PCR Series; BioFact All-in PCR Cloning Kit; BioFact DNA Ladders; BioFact F-Star Taq DNA Polymerase; BioFact Gel & PCR Purification System; BioFact … s m cleaningWebThe total RNA was converted to cDNA with a cDNA synthesis kit (BIOFACT, Korea). Further, SYBR Green (BIOFACT, Korea) was used to specify the expression of the intended genes (Beyrami et al. Citation 2024). The genes were amplified as follows: initial denaturation at 95 °C for 5 min (1X), followed by 35 cycles of 95 °C for 30 s, primer ... s main st fort worth